Ontology highlight
ABSTRACT:
SUBMITTER: Gupta R
PROVIDER: S-EPMC1911201 | biostudies-other | 2007
REPOSITORIES: biostudies-other
Gupta Ronish R Kus Bart B Fladd Christopher C Wasmuth James J Tonikian Raffi R Sidhu Sachdev S Krogan Nevan J NJ Parkinson John J Rotin Daniela D
Molecular systems biology 20070605
Ubiquitin-protein ligases (E3s) are responsible for target recognition and regulate stability, localization or function of their substrates. However, the substrates of most E3 enzymes remain unknown. Here, we describe the development of a novel proteomic in vitro ubiquitination screen using a protein microarray platform that can be utilized for the discovery of substrates for E3 ligases on a global scale. Using the yeast E3 Rsp5 as a test system to identify its substrates on a yeast protein micr ...[more]