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Load-dependent release limits the processive stepping of the tetrameric Eg5 motor.


ABSTRACT: Tetrameric motor proteins of the Kinesin-5 family are essential for eukaryotic cell division. The microscopic mechanism by which Eg5, the vertebrate Kinesin-5, drives bipolar mitotic spindle formation remains unknown. Here we show in optical trapping experiments that full-length Eg5 moves processively and stepwise along microtubule bundles. Interestingly, the force produced by individual Eg5 motors typically reached only approximately 2 pN, one-third of the stall force of Kinesin-1. Eg5 typically detached from microtubules before stalling. This behavior may reflect a regulatory mechanism important for the role of Eg5 in the mitotic spindle.

SUBMITTER: Korneev MJ 

PROVIDER: S-EPMC1914257 | biostudies-other | 2007 Jul

REPOSITORIES: biostudies-other

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