Monitoring of gene knockouts: genome-wide profiling of conditionally essential genes.
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ABSTRACT:
SUBMITTER: Smith LK
PROVIDER: S-EPMC1929150 | biostudies-other | 2007
REPOSITORIES: biostudies-other
Publications
Monitoring of gene knockouts: genome-wide profiling of conditionally essential genes.
Smith Lisa K LK Gomez Maria J MJ Shatalin Konstantin Y KY Lee Hyunwoo H Neyfakh Alexander A AA
Genome biology 20070101 5
We have developed a new microarray-based genetic technique, named MGK (Monitoring of Gene Knockouts), for genome-wide identification of conditionally essential genes. MGK identified bacterial genes that are critical for fitness in the absence of aromatic amino acids, and was further applied to identify genes whose inactivation causes bacterial cell death upon exposure to the bacteriostatic antibiotic chloramphenicol. Our findings suggest that MGK can serve as a robust tool in functional genomics ...[more]
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