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DNA damage-site recognition by lysine conjugates.

ABSTRACT: Simple lysine conjugates are capable of selective DNA damage at sites approximating a variety of naturally occurring DNA-damage patterns. This process transforms single-strand DNA cleavage into double-strand cleavage with a potential impact on gene and cancer therapy or on the design of DNA constructs that require disassembly at a specific location. This study constitutes an example of DNA damage site recognition by molecules that are two orders of magnitude smaller than DNA-processing enzymes and presents a strategy for site-selective cleavage of single-strand nucleotides, which is based on their annealing with two shorter counterstrands designed to recreate the above duplex damage site.

SUBMITTER: Breiner B

PROVIDER: S-EPMC1941802 | biostudies-other | 2007 Aug

REPOSITORIES: biostudies-other

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DNA damage-site recognition by lysine conjugates.

Breiner Boris B Schlatterer Jörg C JC Alabugin Igor V IV Kovalenko Serguei V SV Greenbaum Nancy L NL

Proceedings of the National Academy of Sciences of the United States of America 20070730 32

Simple lysine conjugates are capable of selective DNA damage at sites approximating a variety of naturally occurring DNA-damage patterns. This process transforms single-strand DNA cleavage into double-strand cleavage with a potential impact on gene and cancer therapy or on the design of DNA constructs that require disassembly at a specific location. This study constitutes an example of DNA damage site recognition by molecules that are two orders of magnitude smaller than DNA-processing enzymes a ...[more]

PMID: 17664419

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