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Molecular and microscopic identification of sulfate-reducing bacteria in multispecies biofilms.

ABSTRACT: The population architecture of sulfidogenic biofilms established in anaerobic fixed-bed bioreactors was characterized by selective polymerase chain reaction amplification and fluorescence microscopy. A region of the 16S rRNA common to resident sulfate-reducing bacteria was selectively amplified by the polymerase chain reaction. Sequences of amplification products, with reference to a collection of 16S rRNA sequences representing most characterized sulfate-reducing bacteria, were used to design both general and specific hybridization probes. Fluorescent versions of these probes were used in combination with fluorescence microscopy to visualize specific sulfate-reducing bacterial populations within developing and established biofilms.

SUBMITTER: Amann RI

PROVIDER: S-EPMC195292 | biostudies-other | 1992 Feb

REPOSITORIES: biostudies-other

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Molecular and microscopic identification of sulfate-reducing bacteria in multispecies biofilms.

Amann R I RI Stromley J J Devereux R R Key R R Stahl D A DA

Applied and environmental microbiology 19920201 2

The population architecture of sulfidogenic biofilms established in anaerobic fixed-bed bioreactors was characterized by selective polymerase chain reaction amplification and fluorescence microscopy. A region of the 16S rRNA common to resident sulfate-reducing bacteria was selectively amplified by the polymerase chain reaction. Sequences of amplification products, with reference to a collection of 16S rRNA sequences representing most characterized sulfate-reducing bacteria, were used to design b ...[more]

PMID: 1376982

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