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Identification of Brucella spp. by using the polymerase chain reaction.


ABSTRACT: The application of two synthetic oligonucleotides as probes and as primers in the polymerase chain reaction is presented for a specific, sensitive, and quick identification of Brucella spp. The specific oligonucleotide sequences were chosen on the basis of a 16S rRNA sequence alignment between Brucella abortus and Agrobacterium tumefaciens.

SUBMITTER: Herman L 

PROVIDER: S-EPMC195734 | biostudies-other | 1992 Jun

REPOSITORIES: biostudies-other

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Identification of Brucella spp. by using the polymerase chain reaction.

Herman L L   De Ridder H H  

Applied and environmental microbiology 19920601 6


The application of two synthetic oligonucleotides as probes and as primers in the polymerase chain reaction is presented for a specific, sensitive, and quick identification of Brucella spp. The specific oligonucleotide sequences were chosen on the basis of a 16S rRNA sequence alignment between Brucella abortus and Agrobacterium tumefaciens. ...[more]

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