Ontology highlight
ABSTRACT:
SUBMITTER: Smith RL
PROVIDER: S-EPMC196734 | biostudies-other | 1994 Sep
REPOSITORIES: biostudies-other
Smith R L RL O'Toole J F JF Maguire M E ME Sanders C R CR
Journal of bacteriology 19940901 17
The topology of Escherichia coli diacylglycerol kinase (DAGK) within the cytoplasmic membrane was elucidated by a combined approach involving both multiple aligned sequence analysis and fusion protein experiments. Hydropathy plots of the five prokaryotic DAGK sequences available were uniform in their prediction of three transmembrane segments. The hydropathy predictions were experimentally tested genetically by fusing C-terminal deletion derivatives of DAGK to beta-lactamase and beta-galactosida ...[more]