Ontology highlight
ABSTRACT:
SUBMITTER: Paavilainen S
PROVIDER: S-EPMC202212 | biostudies-other | 1993 Mar
REPOSITORIES: biostudies-other
Paavilainen S S Hellman J J Korpela T T
Applied and environmental microbiology 19930301 3
An intracellular beta-glucosidase was purified from cell extracts of Bacillus circulans subsp. alkalophilus by NAD affinity and high-performance anion-exchange chromatographies. The enzyme was active against a wide range of aryl-beta-glucosides and beta-linked disaccharides. The structural gene for beta-glucosidase was cloned in Escherichia coli. The beta-glucosidase gene consisted of an open reading frame of 1,350 bp encoding a protein of 450 amino acids with a calculated M(r) of 51,303. The en ...[more]