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Use of an Escherichia coli recombinant producing thermostable polyphosphate kinase as an ATP regenerator to produce fructose 1,6-diphosphate.


ABSTRACT: Heat-treated Escherichia coli producing Thermus polyphosphate kinase regenerated ATP by using exogenous polyphosphate. This recombinant could be used as a platform to produce valuable compounds in combination with thermostable phosphorylating or energy-requiring enzymes. In this work, we demonstrated the production of fructose 1,6-diphosphate from fructose and polyphosphate.

SUBMITTER: Iwamoto S 

PROVIDER: S-EPMC2042086 | biostudies-other | 2007 Sep

REPOSITORIES: biostudies-other

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Use of an Escherichia coli recombinant producing thermostable polyphosphate kinase as an ATP regenerator to produce fructose 1,6-diphosphate.

Iwamoto Seishi S   Motomura Kei K   Shinoda Yasuharu Y   Urata Masaaki M   Kato Junichi J   Takiguchi Noboru N   Ohtake Hisao H   Hirota Ryuichi R   Kuroda Akio A  

Applied and environmental microbiology 20070706 17


Heat-treated Escherichia coli producing Thermus polyphosphate kinase regenerated ATP by using exogenous polyphosphate. This recombinant could be used as a platform to produce valuable compounds in combination with thermostable phosphorylating or energy-requiring enzymes. In this work, we demonstrated the production of fructose 1,6-diphosphate from fructose and polyphosphate. ...[more]

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