Ontology highlight
ABSTRACT:
SUBMITTER: Sakai Y
PROVIDER: S-EPMC204756 | biostudies-other | 1993 Jun
REPOSITORIES: biostudies-other
Journal of bacteriology 19930601 11
We have developed a transformation system which uses autonomous replicating plasmids for a methylotrophic yeast, Candida boidinii. Two autonomous replication sequences, CARS1 and CARS2, were newly cloned from the genome of C. boidinii. Plasmids having both a CARS fragment and the C. boidinii URA3 gene transformed C. boidinii ura3 cells to Ura+ phenotype at frequencies of up to 10(4) CFU/micrograms of DNA. From Southern blot analysis, CARS plasmids seemed to exist in polymeric forms as well as in ...[more]