Amplification of the bacA gene confers bacitracin resistance to Escherichia coli.
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ABSTRACT:
SUBMITTER: Cain BD
PROVIDER: S-EPMC204795 | biostudies-other | 1993 Jun
REPOSITORIES: biostudies-other
Publications
Amplification of the bacA gene confers bacitracin resistance to Escherichia coli.
Cain B D BD Norton P J PJ Eubanks W W Nick H S HS Allen C M CM
Journal of bacteriology 19930601 12
An Escherichia coli genomic library was constructed in order to facilitate selection for genes which confer bacitracin resistance through amplification. One of the plasmids from the library, plasmid pXV62, provided a high level of bacitracin resistance for E. coli. Deletion and nucleotide sequence analyses of bacitracin resistance plasmid pXV62 revealed that a single open reading frame, designated the bacA gene, was sufficient for antibiotic resistance. The bacA gene mapped to approximately 67 m ...[more]
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