Ontology highlight
ABSTRACT:
SUBMITTER: Chen J
PROVIDER: S-EPMC205411 | biostudies-other | 1994 May
REPOSITORIES: biostudies-other
Chen J J Brevet A A Lapadat-Tapolsky M M Blanquet S S Plateau P P
Journal of bacteriology 19940501 9
A DNA region carrying lysS, the gene encoding the lysyl-tRNA synthetase, was cloned from the extreme thermophile prokaryote Thermus thermophilus VK-1 and sequenced. The analysis indicated an open reading frame encoding a protein of 492 amino acids. This putative protein has significant homologies to previously sequenced lysyl-tRNA synthetases and displays the three motifs characteristic of class II aminoacyl-tRNA synthetases. The T. thermophilus lysS gene was overexpressed in Escherichia coli by ...[more]