Ontology highlight
ABSTRACT:
SUBMITTER: Suh Y
PROVIDER: S-EPMC205859 | biostudies-other | 1992 Apr
REPOSITORIES: biostudies-other
Journal of bacteriology 19920401 7
Serratia marcescens produces an abundant extracellular metalloprotease. The gene for this protease had previously been cloned and expressed in Escherichia coli, in which no functional protease could be found. However, the protease gene carries the LXGGXGND repeat motif found in alpha-hemolysin and other proteins secreted by homologous systems. Using a dual-plasmid complementation system, we show that the alpha-hemolysin hlyB and hlyD transport determinants are sufficient to allow secretion and a ...[more]