Ontology highlight
ABSTRACT:
SUBMITTER: Verwoert II
PROVIDER: S-EPMC205936 | biostudies-other | 1992 May
REPOSITORIES: biostudies-other
Verwoert I I II Verbree E C EC van der Linden K H KH Nijkamp H J HJ Stuitje A R AR
Journal of bacteriology 19920501 9
The Escherichia coli fabD gene encoding malonyl coenzyme A-acyl carrier protein transacylase (MCT) was cloned by complementation of a thermosensitive E. coli fabD mutant (fabD89). Expression of the fabD gene in an appropriate E. coli expression vector resulted in an accumulation of the MCT protein of up to 10% of total soluble protein, which was accompanied by an approximately 1,000-fold increase in the MCT activity. DNA sequence analysis and expression studies revealed that the fabD gene is par ...[more]