Sequence of the S-layer gene of Thermus thermophilus HB8 and functionality of its promoter in Escherichia coli.
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ABSTRACT:
SUBMITTER: Faraldo MM
PROVIDER: S-EPMC207444 | biostudies-other | 1992 Nov
REPOSITORIES: biostudies-other
Publications
Sequence of the S-layer gene of Thermus thermophilus HB8 and functionality of its promoter in Escherichia coli.
Faraldo M M MM de Pedro M A MA Berenguer J J
Journal of bacteriology 19921101 22
The nucleotide sequence of the slpA gene, which is responsible for the synthesis of the S-layer protein of Thermus thermophilus HB8, is described. This gene is transcribed as a unit in which the coding region is preceded by a 127-base-long leader mRNA sequence. The promoter region is also recognized by the RNA polymerase of Escherichia coli because of the presence of homologous -35 and -10 boxes. Homologies with other promoters from Thermus spp. are also presented. ...[more]
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