Molecular and genetic characterization of an Alcaligenes eutrophus insertion element.
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ABSTRACT:
SUBMITTER: Kung SS
PROVIDER: S-EPMC207540 | biostudies-other | 1992 Dec
REPOSITORIES: biostudies-other
Publications
Molecular and genetic characterization of an Alcaligenes eutrophus insertion element.
Kung S S SS Chen J J Chow W Y WY
Journal of bacteriology 19921201 24
An insertion element, ISAE1, was discovered during the molecular analysis of mutants defective in the autotrophic growth (Aut-) of Alcaligenes eutrophus H1-4, a mitomycin C-generated derivative of strain H1. ISAE1 is 1,313 bp long, has 12-bp nearly perfect inverted terminal repeats, and contains an open reading frame that has a coding capacity of 408 amino acids. Direct repeats of 8 bp were generated by insertion of ISAE1 into chromosomes or plasmids. Most insertion were found in the AT-rich tar ...[more]
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