Analysis of operons encoding 23S rRNA of Clostridium botulinum type A.
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ABSTRACT:
SUBMITTER: East AK
PROVIDER: S-EPMC207558 | biostudies-other | 1992 Dec
REPOSITORIES: biostudies-other
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Analysis of operons encoding 23S rRNA of Clostridium botulinum type A.
East A K AK Thompson D E DE Collins M D MD
Journal of bacteriology 19921201 24
Southern hybridization analysis of Clostridium botulinum type A chromosomal DNA indicated the presence of six copies of the 23S rRNA gene. Fragments of DNA encoding 23S rRNA were amplified by polymerase chain reaction and cloned in Escherichia coli. Three clones examined by restriction enzyme and sequence analysis were found to be derived from different operons. Sequence determination of the entire insert of two clones revealed nine nucleotide changes in the genes coding for 23S rRNA (99.7% sequ ...[more]
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