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Comparative analysis of the replication regions of IncB, IncK, and IncZ plasmids.


ABSTRACT: Minireplicons from the I-complex plasmids R387 (IncK) and pIE545 (IncZ) were constructed, and the nucleotide sequences of their replication regions were compared with that of the B plasmid, pMU720. The coding sequence of the putative replication protein, RepA, of each plasmid was located. RepA of K and B plasmids were homologous, whereas RepA of Z resembled RepA1 of FII plasmid. Sequences upstream of RepA were conserved in the three I-complex plasmids. Group B and Z plasmids were incompatible.

SUBMITTER: Praszkier J 

PROVIDER: S-EPMC207792 | biostudies-other | 1991 Apr

REPOSITORIES: biostudies-other

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Comparative analysis of the replication regions of IncB, IncK, and IncZ plasmids.

Praszkier J J   Wei T T   Siemering K K   Pittard J J  

Journal of bacteriology 19910401 7


Minireplicons from the I-complex plasmids R387 (IncK) and pIE545 (IncZ) were constructed, and the nucleotide sequences of their replication regions were compared with that of the B plasmid, pMU720. The coding sequence of the putative replication protein, RepA, of each plasmid was located. RepA of K and B plasmids were homologous, whereas RepA of Z resembled RepA1 of FII plasmid. Sequences upstream of RepA were conserved in the three I-complex plasmids. Group B and Z plasmids were incompatible. ...[more]

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