Ontology highlight
ABSTRACT:
SUBMITTER: Miller CG
PROVIDER: S-EPMC207971 | biostudies-other | 1991 Jun
REPOSITORIES: biostudies-other
Miller C G CG Miller J L JL Bagga D A DA
Journal of bacteriology 19910601 11
The anaerobically regulated pepT gene of Salmonella typhimurium has been cloned in pBR328. Strains carrying the pepT plasmid, pJG17, overproduce peptidase T by approximately 70-fold. The nucleotide sequence of a 2.5-kb region including pepT has been determined. The sequence codes for a protein of 44,855 Da, consistent with a molecular weight of approximately 46,000 for peptidase T (as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and gel filtration). The N-terminal amin ...[more]