Ontology highlight
ABSTRACT:
SUBMITTER: Beliveau C
PROVIDER: S-EPMC208289 | biostudies-other | 1991 Sep
REPOSITORIES: biostudies-other
Béliveau C C Potvin C C Trudel J J Asselin A A Bellemare G G
Journal of bacteriology 19910901 18
A Streptococcus faecalis genomic bank was obtained by partial digestion with MboI and cloning into the SalI restriction site of pTZ18R. Screening of about 60,000 Escherichia coli transformants for cell wall lysis activity was done by exposing recombinant colonies grown on medium containing lyophilized Micrococcus lysodeikticus cells to chloroform and toluene vapors in order to release proteins. Because this procedure provoked cell death, colonies could not be used directly for transformant recov ...[more]