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Complex character of senS, a novel gene regulating expression of extracellular-protein genes of Bacillus subtilis.

ABSTRACT: The senS gene of Bacillus subtilis, which in high copy number stimulates the expression of several extracellular-protein genes, has been cloned, genetically mapped, and sequenced. The gene codes for a highly charged basic protein containing 65 amino acid residues. The gene is characterized by the presence of a transcription terminator (attenuator) located between the promoter and open reading frame, a strong ribosome-binding site, and a strong transcription terminator at the 3' end of this monocistronic gene. The amino acid sequence of SenS showed partial homology with the N-terminal core binding domain region of bacterial RNA polymerase sigma factors and a helix-turn-helix motif found in DNA-binding proteins. The gene can be deleted without any effect on growth or sporulation.

SUBMITTER: Wang LF

PROVIDER: S-EPMC208689 | biostudies-other | 1990 Apr

REPOSITORIES: biostudies-other

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Complex character of senS, a novel gene regulating expression of extracellular-protein genes of Bacillus subtilis.

Wang L F LF Doi R H RH

Journal of bacteriology 19900401 4

The senS gene of Bacillus subtilis, which in high copy number stimulates the expression of several extracellular-protein genes, has been cloned, genetically mapped, and sequenced. The gene codes for a highly charged basic protein containing 65 amino acid residues. The gene is characterized by the presence of a transcription terminator (attenuator) located between the promoter and open reading frame, a strong ribosome-binding site, and a strong transcription terminator at the 3' end of this monoc ...[more]

PMID: 2108127

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