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Regulatory elements of the raffinose operon: nucleotide sequences of operator and repressor genes.


ABSTRACT: The raffinose (raf) operon is negatively controlled by the specific binding of raf repressor (rafR gene) to raf operator (rafO) DNA. Both rafR and rafO have been sequenced. The 1,011-base-pair rafR gene encodes a 336-amino-acid polypeptide containing an N-terminal helix-turn-helix motif. rafO, as defined by in vivo titration of raf repressor, consists of two nearly identical 18-base-pair palindromes that flank the -35 box of the raf promoter.

SUBMITTER: Aslanidis C 

PROVIDER: S-EPMC208720 | biostudies-other | 1990 Apr

REPOSITORIES: biostudies-other

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Regulatory elements of the raffinose operon: nucleotide sequences of operator and repressor genes.

Aslanidis C C   Schmitt R R  

Journal of bacteriology 19900401 4


The raffinose (raf) operon is negatively controlled by the specific binding of raf repressor (rafR gene) to raf operator (rafO) DNA. Both rafR and rafO have been sequenced. The 1,011-base-pair rafR gene encodes a 336-amino-acid polypeptide containing an N-terminal helix-turn-helix motif. rafO, as defined by in vivo titration of raf repressor, consists of two nearly identical 18-base-pair palindromes that flank the -35 box of the raf promoter. ...[more]

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