Ontology highlight
ABSTRACT:
SUBMITTER: Eggen RI
PROVIDER: S-EPMC208970 | biostudies-other | 1991 Oct
REPOSITORIES: biostudies-other
Eggen R I RI Geerling A C AC Boshoven A B AB de Vos W M WM
Journal of bacteriology 19911001 20
In the acetoclastic methanogen Methanothrix soehngenii, acetate is activated to acetyl coenzyme A by acetyl coenzyme A synthetase (Acs). The acs gene, coding for the single Acs subunit, was isolated from a genomic library of M. soehngenii DNA in Escherichia coli by using antiserum raised against the purified Acs. After introduction in E. coli, the acs gene was expressed, resulting in the production of an immunoreactive protein of 68 kDa, which is approximately 5 kDa smaller than the known size o ...[more]