Ontology highlight
ABSTRACT:
SUBMITTER: Puyet A
PROVIDER: S-EPMC209899 | biostudies-other | 1989 May
REPOSITORIES: biostudies-other
Puyet A A Greenberg B B Lacks S A SA
Journal of bacteriology 19890501 5
The gene encoding the major DNA exonuclease of Streptococcus pneumoniae, exoA, was cloned in a streptococcal host vector system. Its location was determined by subcloning and by insertion mutations. Transfer of a DNA segment containing the gene to an Escherichia coli expression vector showed that exoA was the structural gene for the enzyme and that it was adjacent to its promoter. DNA sequence determination indicated that the gene encoded a protein, ExoA, of molecular weight 31,263. Under hypere ...[more]