Ontology highlight
ABSTRACT:
SUBMITTER: Paschal BM
PROVIDER: S-EPMC2120498 | biostudies-other | 1995 May
REPOSITORIES: biostudies-other
The Journal of cell biology 19950501 4
Protein import into the nucleus is a multistep process that requires the activities of several cytosolic factors. In this study we have purified a cytosolic factor that interacts with the nuclear pore complex glycoprotein p62. Isolation involved biochemical complementation of cytosol depleted of this activity by preadsorption with recombinant p62 and the use of a novel flow cytometry-based assay for quantitation of nuclear import. The purified activity (NTF2) is an apparent dimer of approximatel ...[more]