Demonstration of protein-protein interaction specificity by NMR chemical shift mapping.
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ABSTRACT:
SUBMITTER: Rajagopal P
PROVIDER: S-EPMC2143627 | biostudies-other | 1997 Dec
REPOSITORIES: biostudies-other
Publications
Demonstration of protein-protein interaction specificity by NMR chemical shift mapping.
Rajagopal P P Waygood E B EB Reizer J J Saier M H MH Klevit R E RE
Protein science : a publication of the Protein Society 19971201 12
Chemical shift mapping is becoming a popular method for studying protein-protein interactions in solution. The technique is used to identify putative sites of interaction on a protein surface by detecting chemical shift perturbations in simple (1H, 15N)-HSQC NMR spectra of a uniformly labeled protein as a function of added (unlabeled) target protein. The high concentrations required for these experiments raise questions concerning the possibility for non-specific interactions being detected, the ...[more]
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