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Fas ligand mediates activation-induced cell death in human T lymphocytes.

ABSTRACT: A significant proportion of previously activated human T cells undergo apoptosis when triggered through the CD3/T cell receptor complex, a process termed activation-induced cell death (AICD). Ligation of Fas on activated T cells by either Fas antibodies or recombinant human Fas-ligand (Fas-L) also results in cytolysis. We demonstrate that these two pathways of apoptosis are causally related. Stimulation of previously activated T cells resulted in the expression of Fas-L mRNA and lysis of Fas-positive target cells. Fas-L antagonists inhibited AICD of T cell clones and staphylococcus enterotoxin B (SEB)-specific T cell lines. The data indicate AICD in previously stimulated T cells is mediated by Fas/Fas-L interactions.

SUBMITTER: Alderson MR

PROVIDER: S-EPMC2191813 | biostudies-other | 1995 Jan

REPOSITORIES: biostudies-other

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Fas ligand mediates activation-induced cell death in human T lymphocytes.

Alderson M R MR Tough T W TW Davis-Smith T T Braddy S S Falk B B Schooley K A KA Goodwin R G RG Smith C A CA Ramsdell F F Lynch D H DH

The Journal of experimental medicine 19950101 1

A significant proportion of previously activated human T cells undergo apoptosis when triggered through the CD3/T cell receptor complex, a process termed activation-induced cell death (AICD). Ligation of Fas on activated T cells by either Fas antibodies or recombinant human Fas-ligand (Fas-L) also results in cytolysis. We demonstrate that these two pathways of apoptosis are causally related. Stimulation of previously activated T cells resulted in the expression of Fas-L mRNA and lysis of Fas-pos ...[more]

PMID: 7528780

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