Unknown

Dataset Information

0

The junctional adhesion molecule 3 (JAM-3) on human platelets is a counterreceptor for the leukocyte integrin Mac-1.


ABSTRACT: The recently described junctional adhesion molecules (JAMs) in man and mice are involved in homotypic and heterotypic intercellular interactions. Here, a third member of this family, human JAM-3, was identified and described as a novel counterreceptor on platelets for the leukocyte beta2-integrin Mac-1 (alphaMbeta2, CD11b/CD18). With the help of two monoclonal antibodies, Gi11 and Gi13, against a 43-kD surface glycoprotein on human platelets, a full-length cDNA encoding JAM-3 was identified. JAM-3 is a type I transmembrane glycoprotein containing two Ig-like domains. Although JAM-3 did not undergo homophilic interactions, myelo-monocytic cells adhered to immobilized JAM-3 or to JAM-3-transfected cells. This heterophilic interaction was specifically attributed to a direct interaction of JAM-3 with the beta2-integrin Mac-1 and to a lower extent with p150.95 (alphaXbeta2, CD11c/CD18) but not with LFA-1 (alphaLbeta2, CD11a/CD18) or with beta1-integrins. These results were corroborated by analysis of K562 erythroleukemic cells transfected with different heterodimeric beta2-integrins and by using purified proteins. Moreover, purified JAM-3 or antibodies against JAM-3 blocked the platelet-neutrophil interaction, indicating that platelet JAM-3 serves as a counterreceptor for Mac-1 mediating leukocyte-platelet interactions. JAM-3 thereby provides a novel molecular target for antagonizing interactions between vascular cells that promote inflammatory vascular pathologies such as in atherothrombosis.

SUBMITTER: Santoso S 

PROVIDER: S-EPMC2194005 | biostudies-other | 2002 Sep

REPOSITORIES: biostudies-other

altmetric image

Publications

The junctional adhesion molecule 3 (JAM-3) on human platelets is a counterreceptor for the leukocyte integrin Mac-1.

Santoso Sentot S   Sachs Ulrich J H UJ   Kroll Hartmut H   Linder Monica M   Ruf Andreas A   Preissner Klaus T KT   Chavakis Triantafyllos T  

The Journal of experimental medicine 20020901 5


The recently described junctional adhesion molecules (JAMs) in man and mice are involved in homotypic and heterotypic intercellular interactions. Here, a third member of this family, human JAM-3, was identified and described as a novel counterreceptor on platelets for the leukocyte beta2-integrin Mac-1 (alphaMbeta2, CD11b/CD18). With the help of two monoclonal antibodies, Gi11 and Gi13, against a 43-kD surface glycoprotein on human platelets, a full-length cDNA encoding JAM-3 was identified. JAM  ...[more]

Similar Datasets

| S-EPMC3938150 | biostudies-literature
| S-EPMC5477519 | biostudies-literature
2023-08-31 | GSE230828 | GEO
| S-EPMC10215618 | biostudies-literature
| S-EPMC5542900 | biostudies-literature
| S-EPMC3089572 | biostudies-literature
| S-EPMC1237098 | biostudies-literature
| S-EPMC2206366 | biostudies-literature
| S-EPMC2366836 | biostudies-literature
| S-EPMC4273560 | biostudies-other