Characterization of cellular chemical dynamics using combined microfluidic and Raman techniques.
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ABSTRACT: The integration of a range of technologies including microfluidics, surface-enhanced Raman scattering and confocal microspectroscopy has been successfully used to characterize in situ single living CHO (Chinese hamster ovary) cells with a high degree of spatial (in three dimensions) and temporal (1 s per spectrum) resolution. Following the introduction of a continuous flow of ionomycin, the real time spectral response from the cell was monitored during the agonist-evoked Ca(2+) flux process. The methodology described has the potential to be used for the study of the cellular dynamics of a range of signalling processes.
SUBMITTER: Zhang X
PROVIDER: S-EPMC2226000 | biostudies-other | 2008 Feb
REPOSITORIES: biostudies-other
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