Ontology highlight
ABSTRACT:
SUBMITTER: Fujita S
PROVIDER: S-EPMC228076 | biostudies-other | 1995 Apr
REPOSITORIES: biostudies-other
Fujita S S Lasker B A BA Lott T J TJ Reiss E E Morrison C J CJ
Journal of clinical microbiology 19950401 4
We developed a microtitration plate enzyme immunoassay to detect PCR-amplified DNA from Candida species. Nucleotide sequences derived from the internal transcribed spacer (ITS) region of fungal rDNA were used to develop species-specific oligonucleotide probes for Candida albicans, C. tropicalis, C. parapsilosis, and C. krusei. No cross-hybridization was detected with any other fungal, bacterial, or human DNAs tested. In contrast, a C. (Torulopsis) glabrata probe cross-reacted with Saccharomyces ...[more]