Ontology highlight
ABSTRACT:
SUBMITTER: Anderson B
PROVIDER: S-EPMC228412 | biostudies-other | 1995 Sep
REPOSITORIES: biostudies-other
Journal of clinical microbiology 19950901 9
A library of Bartonella (Rochalimaea) henselae DNA was constructed in the cloning vector lambda ZAPII and screened for expression of antigenic proteins by using a pool of sera from patients who had been diagnosed with cat scratch disease (CSD) and had antibodies to Bartonella spp., as determined by indirect fluorescent-antibody (IFA) assay. Ten immunoreactive phages were subcloned as recombinant plasmids by in vivo excision. All 10 recombinants expressed a protein of approximately 17 kDa when th ...[more]