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Identification and analysis of a functional human homolog of the SPT4 gene of Saccharomyces cerevisiae.

ABSTRACT: Spt4p is a nonhistone protein of Saccharomyces cerevisiae that is believed to be required for normal chromatin structure and transcription. In this work we describe the isolation and analysis of a human gene, SUPT4H, that encodes a predicted protein 42% identical to Spt4p. When expressed in S. cerevisiae, SUPT4H complemented all spt4 mutant phenotypes. In human cells SUPT4H encodes a nuclear protein that is expressed in all tissues tested. In addition, hybridization analyses suggest that an SUPT4H-related gene is also present in mice. SUPT4H was localized to human chromosome 17 by PCR analysis of a human-rodent somatic cell hybrid panel. Thus, like other proteins that are components of or control the structure of chromatin, Spt4p appears to be conserved from S. cerevisiae to mammals.

SUBMITTER: Hartzog GA

PROVIDER: S-EPMC231277 | biostudies-other | 1996 Jun

REPOSITORIES: biostudies-other

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Identification and analysis of a functional human homolog of the SPT4 gene of Saccharomyces cerevisiae.

Hartzog G A GA Basrai M A MA Ricupero-Hovasse S L SL Hieter P P Winston F F

Molecular and cellular biology 19960601 6

Spt4p is a nonhistone protein of Saccharomyces cerevisiae that is believed to be required for normal chromatin structure and transcription. In this work we describe the isolation and analysis of a human gene, SUPT4H, that encodes a predicted protein 42% identical to Spt4p. When expressed in S. cerevisiae, SUPT4H complemented all spt4 mutant phenotypes. In human cells SUPT4H encodes a nuclear protein that is expressed in all tissues tested. In addition, hybridization analyses suggest that an SUPT ...[more]

PMID: 8649394

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