Unknown

Dataset Information

0

P53-dependent association between cyclin G and the B' subunit of protein phosphatase 2A.


ABSTRACT: We and others previously showed that cyclin G is a transcriptional target of the p53 tumor suppressor protein. However, cellular proteins which might form a complex with cyclin G have not yet been identified. To gain insight into the biological role of cyclin G, we used the yeast two-hybrid screen and isolated two mouse cDNAs encoding cyclin G-interacting proteins. Interestingly, both positive cDNAs encoded B' regulatory subunits of protein phosphatase 2A (PP2A). One clone encodes B'alpha, while the other clone codes for a new member of the B' family, B'beta. B'beta is 70% identical to other members of the B' family. B'alpha associated both in vitro and in vivo with cyclin G but not with the other mammalian cyclins. Furthermore, cyclin G formed a complex with B'alpha only after induction of p53 in p53 temperature-sensitive cell lines. These results indicate that cyclin G forms a specific complex with the B' subunit of PP2A and that complex formation is regulated by p53. Potential roles for the cyclin G-B' complex in p53-mediated pathways are discussed.

SUBMITTER: Okamoto K 

PROVIDER: S-EPMC231661 | biostudies-other | 1996 Nov

REPOSITORIES: biostudies-other

altmetric image

Publications

p53-dependent association between cyclin G and the B' subunit of protein phosphatase 2A.

Okamoto K K   Kamibayashi C C   Serrano M M   Prives C C   Mumby M C MC   Beach D D  

Molecular and cellular biology 19961101 11


We and others previously showed that cyclin G is a transcriptional target of the p53 tumor suppressor protein. However, cellular proteins which might form a complex with cyclin G have not yet been identified. To gain insight into the biological role of cyclin G, we used the yeast two-hybrid screen and isolated two mouse cDNAs encoding cyclin G-interacting proteins. Interestingly, both positive cDNAs encoded B' regulatory subunits of protein phosphatase 2A (PP2A). One clone encodes B'alpha, while  ...[more]

Similar Datasets

| S-EPMC4680974 | biostudies-literature
| S-EPMC1887582 | biostudies-literature
| S-EPMC3327649 | biostudies-literature
| S-EPMC2900437 | biostudies-literature
| S-EPMC3900686 | biostudies-literature
| S-EPMC10915630 | biostudies-literature
| S-EPMC6277666 | biostudies-other
| S-EPMC1815292 | biostudies-literature
| S-EPMC2770260 | biostudies-literature
2016-08-01 | E-GEOD-66839 | biostudies-arrayexpress