Human herpesvirus 6B origin: sequence diversity, requirement for two binding sites for origin-binding protein, and enhanced replication from origin multimers.
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ABSTRACT:
SUBMITTER: Dewhurst S
PROVIDER: S-EPMC237107 | biostudies-other | 1994 Oct
REPOSITORIES: biostudies-other
Publications
Human herpesvirus 6B origin: sequence diversity, requirement for two binding sites for origin-binding protein, and enhanced replication from origin multimers.
Dewhurst S S Krenitsky D M DM Dykes C C
Journal of virology 19941001 10
A previously identified human herpesvirus 6B (HHV-6B) origin of DNA replication contains two binding sites for the origin-binding protein (OBPH6B). We have investigated the functional significance of these sites by determining the replication efficiencies of mutated origin sequences, using a transient replication assay. The results indicate that both sites are required for DNA replication. In addition, we have tested the functional consequences of linear sequence amplifications in the origin. Th ...[more]
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