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New and sensitive assay for determining Pseudomonas aeruginosa metallo-beta-lactamase resistance to imipenem.

ABSTRACT: We present an imipenem lysate metallo-beta-lactamase (MBL) functional assay. This assay eliminates false-positive results due to the bactericidal effects of EDTA, can be performed with inexpensive reagents available in most laboratories, and is as accurate as the MBL Etest. It is appropriate for both high-accuracy screens and laboratories in developing countries with limited resources.

SUBMITTER: Tan J

PROVIDER: S-EPMC2395087 | biostudies-other | 2008 May

REPOSITORIES: biostudies-other

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New and sensitive assay for determining Pseudomonas aeruginosa metallo-beta-lactamase resistance to imipenem.

Tan Jingyuan J Pitout Johann D D JD Guttman David S DS

Journal of clinical microbiology 20080312 5

We present an imipenem lysate metallo-beta-lactamase (MBL) functional assay. This assay eliminates false-positive results due to the bactericidal effects of EDTA, can be performed with inexpensive reagents available in most laboratories, and is as accurate as the MBL Etest. It is appropriate for both high-accuracy screens and laboratories in developing countries with limited resources. ...[more]

PMID: 18337388

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