Methods in molecular cardiology: DHPLC mutation detection analysis.
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ABSTRACT:
SUBMITTER: Jongbloed RJ
PROVIDER: S-EPMC2497274 | biostudies-other | 2005 Jan
REPOSITORIES: biostudies-other
Publications
Methods in molecular cardiology: DHPLC mutation detection analysis.
Jongbloed R J E RJ Smeets H H Doevendans P A PA van den Wijngaard A A
Netherlands heart journal : monthly journal of the Netherlands Society of Cardiology and the Netherlands Heart Foundation 20050101 1
An increasing number of mutations have been identified in genes involved in cardiac disorders which has led to novel insights in the pathophysiology of inherited cardiac diseases. As a result of these findings, techniques specialised in automated high-throughput analysis are implemented to handle the increasing number of diagnostic genetic requests. Denaturing high-performance liquid chromatography (DHPLC) is one such novel technique that fulfils the criteria of speed, sensitivity and accuracy. ...[more]
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