Ontology highlight
ABSTRACT:
SUBMITTER: Manneville JB
PROVIDER: S-EPMC2579358 | biostudies-other | 2008 Nov
REPOSITORIES: biostudies-other
Proceedings of the National Academy of Sciences of the United States of America 20081030 44
Cytoplasmic coat proteins are required for cargo selection and budding of tubulovesicular transport intermediates that shuttle between intracellular compartments. To better understand the physical parameters governing coat assembly and coat-induced membrane deformation, we have reconstituted the Arf1-dependent assembly of the COPI coat on giant unilamellar vesicles by using fluorescently labeled Arf1 and coatomer. Membrane recruitment of Arf1-GTP occurs exclusively on disordered lipid domains an ...[more]