Ontology highlight
ABSTRACT:
SUBMITTER: Namboori SC
PROVIDER: S-EPMC2597562 | biostudies-other | 2008 Oct
REPOSITORIES: biostudies-other
Namboori Seema C SC Graham David E DE
Analytical biochemistry 20080627 1
The Methanococcus maripaludis MMP0352 protein belongs to an oxidoreductase family that has been proposed to catalyze the NAD(+)-dependent oxidation of the 3'' position of uridine diphosphate N-acetyl-D-glucosamine (UDP-GlcNAc), forming a 3-hexulose sugar nucleotide. The heterologously expressed MMP0352 protein was purified and shown to efficiently catalyze UDP-GlcNAc oxidation, forming one NADH equivalent. This enzyme was used to develop a fixed endpoint fluorometric method to analyze UDP-GlcNAc ...[more]