Ontology highlight
ABSTRACT:
SUBMITTER: Xu R
PROVIDER: S-EPMC2615090 | biostudies-other | 2009 Jan
REPOSITORIES: biostudies-other
Xu Ren R Nelson Celeste M CM Muschler John L JL Veiseh Mandana M Vonderhaar Barbara K BK Bissell Mina J MJ
The Journal of cell biology 20090101 1
Epithelial cells, once dissociated and placed in two-dimensional (2D) cultures, rapidly lose tissue-specific functions. We showed previously that in addition to prolactin, signaling by laminin-111 was necessary to restore functional differentiation of mammary epithelia. Here, we elucidate two additional aspects of laminin-111 action. We show that in 2D cultures, the prolactin receptor is basolaterally localized and physically segregated from its apically placed ligand. Detachment of the cells ex ...[more]