Dataset Information

Bursaphelenchus anatolius n. sp. (Nematoda: Parasitaphelenchidae), an Associate of Bees in the Genus Halictus.

ABSTRACT: Bursaphelenchus anatolius n. sp., a phoretic associate of Halictus bees from Ankara, Turkey, is described and illustrated. Bursaphelenchus anatolius n. sp. is closest to B. kevini, which is phoretically associated with Halictus bees from the Pacific Northwest. Bursaphelenchus anatolius n. sp. and B. kevini appear to be sister taxa based upon several shared morphological features, similar life histories involving phoresy with soil-dwelling Halictus bees, and molecular analysis of the near-full-length small subunit rDNA, D2D3 expansion segments of the large subunit rDNA, and partial mitochondrial DNA COI. Bursaphelenchus anatolius n. sp. can be differentiated from all other species of Bursaphelenchus based upon spicule morphology. The paired spicules are uniquely shaped and ventrally recurved, and both B. anatolius n. sp. and B. kevini possess extending flaps that open when the spicules are protracted beyond the cloaca. Population growth of B. anatolius n. sp. was measured at 23 degrees C in the laboratory on cultures of the fungus Monilinia fructicola grown on lactic acid-treated, 5% glycerol-supplemented potato dextrose agar. Nematode population densities rapidly increased from 110 to about 110,000/9-cm-diam. dish within 21 days.

SUBMITTER: Giblin-Davis RM

PROVIDER: S-EPMC2620966 | biostudies-other | 2005 Sep

REPOSITORIES: biostudies-other

ACCESS DATA

Publications

Bursaphelenchus anatolius n. sp. (Nematoda: Parasitaphelenchidae), an Associate of Bees in the Genus Halictus.

Giblin-Davis Robin M RM Hazir Selçuk S Center Barbara J BJ Ye Weimin W Keskin Nevin N Thorp Robbin W RW Thomas W Kelley WK

Journal of nematology 20050901 3

Bursaphelenchus anatolius n. sp., a phoretic associate of Halictus bees from Ankara, Turkey, is described and illustrated. Bursaphelenchus anatolius n. sp. is closest to B. kevini, which is phoretically associated with Halictus bees from the Pacific Northwest. Bursaphelenchus anatolius n. sp. and B. kevini appear to be sister taxa based upon several shared morphological features, similar life histories involving phoresy with soil-dwelling Halictus bees, and molecular analysis of the near-full-le ...[more]

PMID: 19262882

Similar Datasets

Project description:BackgroundThe genus Aegialoalaimus de Man, 1907 includes 12 nominal species, of which three species are considered valid, two species were transferred to other genera and seven species have uncertain taxonomic status.New informationNew species, Aegialoalaimus bratteni sp. n. was found in Skagerrak off the west coast of Sweden. It is particularly characterized by the 1.5-1.8 mm long body, short papilliform cephalic sensilla, excretory pore opening just posterior to nerve ring level, spicules that are straight in shape, supplements and gubernaculum absent, separating it from other valid species of the genus. It can be further differentiated from Aegialoalaimus elegans in having longer body (1.5-1.8 mm in A. bratteni sp. n. vs 0.8-1.3 mm in A. elegans), shape and size of spicules (straight and 22-29 µm long in A. bratteni sp. n. vs arcuate and 34 µm long in A. elegans), absence of precloacal supplements (vs seven-eight in A. elegans), absence of gubernaculum (vs present in A. elegans); from A. setosa in having shorter tail (c´=2.6-3.1 in A. bratteni sp. n. vs c´=4.2 in A. setosa), shorter cephalic sensilla (0.5-1.0 µm in A. bratteni sp. n. vs 9 µm in A. setosa), shape and size of spicules (straight and 22-29 µm long in A. bratteni sp. n. vs arcuate and 40-45 µm long in A. setosa), absence of precloacal supplements (vs eight in A. setosa), absence of gubernaculum (vs present in A. setosa); from A. leptosoma in having longer body (1.5-1.8 mm in A. bratteni sp. n. vs 0.5-0.7 mm in A. leptosoma) and other measurements, shape of spicules (straight in A. bratteni sp. n. vs arcuate in A. leptosoma), absence of precloacal supplements (vs three-five in A. leptosoma), absence of gubernaculum (vs present in A. leptosoma). Type specimens of Aegialoalaimus cylindricauda Allgén, 1933 and A. paratenuicaudatus Allgén, 1959 are redescribed and taxonomic status of these two species is re-evaluated. A taxonomic review, tabular compendium and identification key for species of the genus Aegialoalaimus are also given.

Project description:BackgroundThe genus Marshallagia (Family Haemonchidae, subfamily Ostertagiinae) contains multiple species of nematodes parasitising the abomasum (or duodenum) of ruminants, in particular of Caprinae. Male specimens have been described to be polymorphic with the frequent/major morphotype initially described in the genus Marshallagia while the minor/rare morphotype was initially often placed in the genus Grossospicularia. Due to common morphological features, certain pairs of morphotypes were suggested to belong to the same species such as Marshallagia marshalli/M. occidentalis. However, molecular evidence to confirm these pairs of morphotypes belonging to the same species is missing.MethodsIn the present study, Marshallagia sp. were collected from domestic sheep in Uzbekistan. Male specimens were morphologically described with particular emphasis on the structure of the bursa copulatrix. After DNA isolation from morphologically identified specimens, PCRs targeting the ribosomal internal transcribed spacer 2 (ITS2) and mitochondrial cytochrome c oxidase subunit 1 (cox1) regions were conducted. After Sanger sequencing, maximum likelihood phylogenetic analyses and pairwise identities between sequences were calculated.ResultsThe major morphotypes of M. marshalli, M. schumakovitschi and M. uzbekistanica and the minor morphotypes M. occidentalis, M. trifida and M. sogdiana were identified and their morphology was documented in detail. ITS2 sequences showed little variation and did not allow diagnosing species. In contrast, phylogenetic analysis of cox1 sequences identified highly supported clusters and verified that M. marshalli, M. occidentalis and M. uzbekistanica are different morphotypes of the species M. marshalli while M. schumakovitschi and M. trifida represent distinct morphotypes of M. trifida. For M. sogdiana no corresponding major morphotype could be identified in the present study. Due to a large barcoding gap, comparison of cox1 sequences in terms of percent identity was sufficient to reliably assign the sequences to a particular species without phylogenetic analysis.ConclusionsThe data presented here create a framework that will allow the classification of other members of the genus in the future and underline that parallel morphological and molecular analysis of specimens is crucial to improve the taxonomy of polymorphic species.

Dataset Information

Bursaphelenchus anatolius n. sp. (Nematoda: Parasitaphelenchidae), an Associate of Bees in the Genus Halictus.

Publications

Bursaphelenchus anatolius n. sp. (Nematoda: Parasitaphelenchidae), an Associate of Bees in the Genus Halictus.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets