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Nested polymerase chain reaction for amplification of the Cryptosporidium oocyst wall protein gene.

ABSTRACT: We developed a sensitive nested polymerase chain reaction procedure for the Cryptosporidium oocyst wall protein (COWP) gene. Amplification and genotyping were successful in 95.2% of 1,680 fecal samples, 77.6% by the unnested and 17.6% by the nested COWP procedure. The COWP gene was amplified from 2,128 fecal samples: 71 from livestock animals and 2,057 from humans. This series included 706 cases from seven drinking water-associated outbreaks and 51 cases from five swimming pool-associated outbreaks, as well as 1,300 sporadic cases.

SUBMITTER: Pedraza-Diaz S

PROVIDER: S-EPMC2631669 | biostudies-other | 2001 Jan-Feb

REPOSITORIES: biostudies-other

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Nested polymerase chain reaction for amplification of the Cryptosporidium oocyst wall protein gene.

Pedraza-Díaz S S Amar C C Nichols G L GL McLauchlin J J

Emerging infectious diseases 20010101 1

We developed a sensitive nested polymerase chain reaction procedure for the Cryptosporidium oocyst wall protein (COWP) gene. Amplification and genotyping were successful in 95.2% of 1,680 fecal samples, 77.6% by the unnested and 17.6% by the nested COWP procedure. The COWP gene was amplified from 2,128 fecal samples: 71 from livestock animals and 2,057 from humans. This series included 706 cases from seven drinking water-associated outbreaks and 51 cases from five swimming pool-associated outbre ...[more]

PMID: 11266294

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