Ontology highlight
ABSTRACT:
SUBMITTER: Arezi B
PROVIDER: S-EPMC2632894 | biostudies-other | 2009 Feb
REPOSITORIES: biostudies-other
Nucleic acids research 20081204 2
In an effort to increase the thermostability of Moloney Murine Leukemia Virus reverse transcriptase (MMLV RT), we screened random and site-saturation libraries for variants that show increased resistance to thermal inactivation. We discovered five mutations E69K, E302R, W313F, L435G and N454K that collectively increase the half-life of MMLV RT at 55 degrees C from less than 5 min to approximately 30 min in the presence of template-primer. In addition, these mutations alter the thermal profile by ...[more]