Ontology highlight
ABSTRACT:
SUBMITTER: Pires-Alves M
PROVIDER: S-EPMC2649687 | biostudies-other | 2009 Mar
REPOSITORIES: biostudies-other
Pires-Alves Melissa M Ho Mengfei M Aberle Karla K KK Janda Kim D KD Wilson Brenda A BA
Toxicon : official journal of the International Society on Toxinology 20090301 4
The light chain of botulinum neurotoxin A (BoNT/A-LC) is a zinc-metalloprotease that requires two extended exosites for optimal substrate binding and recognition of its intracellular target SNAP25. CFP and YFP connected through SNAP25 peptide (141-206) containing both exosites (CsY) has been used in a FRET-based assay for BoNT/A. To further improve the FRET efficiency in this BoNT/A substrate for in vitro high-throughput assays, we explored the feasibility of enhancing the capture of CFP emissio ...[more]