Expression of feline immunodeficiency virus gag and env precursor proteins in Spodoptera frugiperda cells and their use in immunodiagnosis.
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ABSTRACT:
SUBMITTER: Verschoor EJ
PROVIDER: S-EPMC265759 | biostudies-other | 1993 Sep
REPOSITORIES: biostudies-other
Publications
Expression of feline immunodeficiency virus gag and env precursor proteins in Spodoptera frugiperda cells and their use in immunodiagnosis.
Verschoor E J EJ van Vliet A L AL Egberink H F HF Hesselink W W Horzinek M C MC de Ronde A A
Journal of clinical microbiology 19930901 9
The gag and env genes of the feline immunodeficiency virus strain UT113 were cloned into a baculovirus transfer vector. The recombinant plasmids were used to create recombinant baculoviruses that expressed either the gag or the env precursor protein in insect cells (Sf9 cells). Leader sequence cleavage occurred in Sf9 cells expressing the envelope precursor, but further processing was not observed. Crude lysates of insect cells infected with the wild-type baculovirus or with the recombinant viru ...[more]
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