Ontology highlight
ABSTRACT:
SUBMITTER: McLaughlin GL
PROVIDER: S-EPMC269744 | biostudies-other | 1991 Feb
REPOSITORIES: biostudies-other
Journal of clinical microbiology 19910201 2
By using hybridization at low C0t values, a genomic library on Naegleria fowleri was screened for clones containing repetitive DNA. Partial sequence information from a repetitive clone, Nf9, showed sequence homologies with the mitochondrial ATPase 6 subunit from yeasts and other organisms. Synthetic DNA primers were selected and tested in amplification reactions. Nonstringent hybridization conditions were defined which allowed amplification of N. fowleri DNA and reduced amplification of DNA from ...[more]