Unknown

Dataset Information

0

High expression of HSP47 in ulcerative colitis-associated carcinomas: proteomic approach.


ABSTRACT: BACKGROUND: Ulcerative colitis (UC) is a chronic relapsing inflammatory bowel disease, known to be associated with a markedly increased risk of colorectal carcinoma development. METHODS: Using proteomic analysis with two-dimensional gel electrophoresis and mass spectrometry, differentially expressed proteins were assessed between UC-associated cancer and sporadic colon cancer cell lines. Western blot and immunostaining were performed for confirming the expression. RESULTS: Heat-shock protein of 47 kDa (HSP47) was identified as one of the proteins expressed more highly in UC-associated cancer cell lines, and an immunohistochemical examination confirmed significantly higher levels of HSP47 in UC-associated colon cancers than in sporadic counterparts, the expression increasing with a progression of neoplastic lesions. Heat-shock protein of 47 kDa was further found to be coexpressed with type I collagen in the cytoplasm, and both HSP47 and type I collagen were released from cultured cells into the culture medium. CONCLUSION: These results suggest that overexpression of HSP47 is a unique characteristic of UC-associated carcinoma related to type I collagen synthesis, with possible clinical applications.

SUBMITTER: Araki K 

PROVIDER: S-EPMC2720226 | biostudies-other | 2009 Aug

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC8154647 | biostudies-literature
| S-EPMC1727192 | biostudies-other
| S-EPMC7587454 | biostudies-literature
| S-EPMC8672077 | biostudies-literature
| 2651254 | ecrin-mdr-crc
| S-EPMC7719760 | biostudies-literature
| S-EPMC5931983 | biostudies-literature
| S-EPMC6487890 | biostudies-literature
2020-12-17 | PXD020244 | Pride
| S-EPMC4603667 | biostudies-literature