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Selective probing of mRNA expression levels within a living cell.


ABSTRACT: We report on a selective and nondestructive measurement of mRNA (messenger ribonucleic acid) expression levels within a living cell. We first modify an atomic force microscope tip to create a tapered nanoscale coaxial cable. Application of an ac (alternating potential) between the inner and outer electrodes of this cable creates a dielectrophoretic force attracting mRNA molecules toward the tip-end which is pretreated with gene specific primers. We selectively extracted and analyzed both high ( approximately 2500) and extremely low (11 0) copy number mRNA from a living cell mRNA in less than 10 s.

SUBMITTER: Nawarathna D 

PROVIDER: S-EPMC2749444 | biostudies-other | 2009 Aug

REPOSITORIES: biostudies-other

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