Ontology highlight
ABSTRACT:
SUBMITTER: Revil T
PROVIDER: S-EPMC2755870 | biostudies-other | 2009 Aug
REPOSITORIES: biostudies-other
Revil Timothée T Pelletier Jordan J Toutant Johanne J Cloutier Alexandre A Chabot Benoit B
The Journal of biological chemistry 20090611 32
The Bcl-x pre-mRNA is alternatively spliced to produce the anti-apoptotic Bcl-x(L) and the pro-apoptotic Bcl-x(S) isoforms. By performing deletion mutagenesis on a human Bcl-x minigene, we have identified a novel exonic element that controls the use of the 5' splice site of Bcl-x(S). The proximal portion of this element acts as a repressor and is located downstream of an enhancer. Further mutational analysis provided a detailed topological map of the regulatory activities revealing a sharp trans ...[more]