Ontology highlight
ABSTRACT:
SUBMITTER: Ding JB
PROVIDER: S-EPMC2756148 | biostudies-other | 2009 Aug
REPOSITORIES: biostudies-other
Ding Jun B JB Takasaki Kevin T KT Sabatini Bernardo L BL
Neuron 20090801 4
Two-photon laser scanning microscopy (2PLSM) has allowed unprecedented fluorescence imaging of neuronal structure and function within neural tissue. However, the resolution of this approach is poor compared to that of conventional confocal microscopy. Here, we demonstrate supraresolution 2PLSM within brain slices. Imaging beyond the diffraction limit is accomplished by using near-infrared (NIR) lasers for both pulsed two-photon excitation and continuous wave stimulated emission depletion (STED). ...[more]