Ontology highlight
ABSTRACT:
SUBMITTER: Ballarino M
PROVIDER: S-EPMC2756881 | biostudies-other | 2009 Oct
REPOSITORIES: biostudies-other
Ballarino Monica M Pagano Francesca F Girardi Erika E Morlando Mariangela M Cacchiarelli Davide D Marchioni Marcella M Proudfoot Nicholas J NJ Bozzoni Irene I
Molecular and cellular biology 20090810 20
The first step in microRNA (miRNA) biogenesis occurs in the nucleus and is mediated by the Microprocessor complex containing the RNase III-like enzyme Drosha and its cofactor DGCR8. Here we show that the 5'-->3' exonuclease Xrn2 associates with independently transcribed miRNAs and, in combination with Drosha processing, attenuates transcription in downstream regions. We suggest that, after Drosha cleavage, a torpedo-like mechanism acts on nascent long precursor miRNAs, whereby Xrn2 exonuclease d ...[more]